›› 2011, Vol. 23 ›› Issue (3): 209-212.doi: 10.3969/j.issn.1004-616x.2011.03.012

• 论著 • Previous Articles     Next Articles

Toxic effects of the extracted solution of disposable chopstick toothpick or cup in murine L-929 cells

CHEN Si-fan1，2，LI Wen-xue1，JIA Hui-ying2，CHEN Jian-ling1，2，YANG Guang-yu1，ZHU Wei1，2，*   

  1. 1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510080; 2. School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong, China
  • Received:2010-12-22 Revised:2011-01-19 Online:2011-05-30 Published:2011-05-30
  • Contact: ZHU Wei

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Abstract: To evaluate the toxic effects of the extracted solution of disposable chopstick， toothpick or cup in murine L-929 cells. METHODS：According to the national standards，we prepared cell culture medium with the extracted solution of disposable chopsticks，toothpicks or cups. The cell morphology of murine L-929 cells was examined microscopically and cell viability was detected with WST-1 staining method. The detached cell ratios and the relative growth rate of L-929 cells were calculated. RESULTS：Compared with the control，after treatment for 4 days or 7 days，cells in the experimental groups showed signs of apoptosis or necrosis. The detached cell ratios were increased significantly (P<0.05)，while cell viability and relative growth rate declined significantly (P<0.05). CONCLUSION： Treatments with extracted solution of disposable chopstick，toothpick or cup could affect cell growth and proliferation，thus probably possess potential toxic effects.

Key words: toothpick, disposable chopstick, disposable cup, cytotoxicity